Fig. 4: E47 positively correlates with ΔNp63α expression and its high expression is associated with poor overall survival in human lung squamous cell carcinoma.

A Adenocarcinoma A549 and SCC H292, TE1, FaDu, KYSE450, KYSE150, or HONE1 cells were subjected to Western blot analyses. B The Oncomine datasets “Su_Esophagus” and “Bild_Lung” were used to analyze Pearson correlation of E47 (E2A) and p63 expression in ESCC and LUSC. C–D Consecutive tissue microarray slides derived from human lung squamous cell carcinoma (HLug-S030PG02) were subjected to IHC analysis for Pearson correlation of E47 (E2A) and p63. p63 antibody used here can recognize all isoforms of p63 (CY5659, Abways, Shanghai, China). Representative images were shown. Data were quantified by average optical density (AOD). Scale bar = 100 μm. E Kaplan–Meier plots of overall survival of human squamous cell carcinoma patients were stratified by the E2A mRNA expression levels in the patient tumor samples. F A working model depicting that the opposite roles of E47 on cell proliferation in adenocarcinoma and squamous cell carcinoma. E47 can function as a pro-oncogene by targeting ΔNp63 promoter to promote it transcription, resulting in promoting cell proliferation and tumor growth in lung squamous cell carcinoma.