Fig. 4: ATF3 regulates decidualization mediated by FOXO1. | Cell Death & Disease

Fig. 4: ATF3 regulates decidualization mediated by FOXO1.

From: ATF3 deficiency impairs the proliferative–secretory phase transition and decidualization in RIF patients

Fig. 4

A hESCs were treated with Ad-LacZ or Ad-ATF3-His for 48 h and transfected with IRS-luc and Rinella for dual-luciferase assays. ****P < 0.0001. B–D hESCs were transfected with Ad-ATF3-his (0 or 40 MOI) or Ad-LacZ. si-FOXO1 was used to knockdown the expression of FOXO1, and prolactin secretion was measured by an enzyme-linked fluorescent assay (ELFA). *P < 0.05. Immunofluorescence was performed to analyze the morphological transformation of hESCs. Scale bar = 50 μm. E hESCs were transfected with Ad-ATF3-his (0, 20, or 40 MOI) or Ad-LacZ. FOXO1 and His protein expression levels were measured by western blot.

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