Fig. 4: NRP1 knockdown counteracted the aggressive phenotype induced by HIF-1α. | Cell Death & Disease

Fig. 4: NRP1 knockdown counteracted the aggressive phenotype induced by HIF-1α.

From: HIF-1α promoted vasculogenic mimicry formation in lung adenocarcinoma through NRP1 upregulation in the hypoxic tumor microenvironment

Fig. 4

A Effects of HIF-1α and NRP1 overexpression on the tube formation abilities of the A549 and SPC-A1 cell lines. B Transwell assay of the invasion and migration ability of LUAD cells after NRP1 or HIF-1α overexpression followed by NRP1 knockdown (B, ×40; scale bars, 100 μm). C Wound healing assay of the migratory ability of LUAD cells after NRP1 or HIF-1α overexpression followed by NRP1 knockdown (B, ×40; scale bars, 100 μm). D Western blot assay of HIF-1α, NRP1, VE-cadherin, MMP-2, Vimentin, and β-actin expression levels. E The mRNA expression levels of HIF-1α, NRP1, and VE-cadherin in the A549 and SPC-A1 cell lines after NRP1 or HIF-1α overexpression followed by NRP1 knockdown. *p < 0.05.

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