Fig. 2: TNFα induces apoptosis in HeLa cells but is resisted by FHL124 cells. | Cell Death & Disease

Fig. 2: TNFα induces apoptosis in HeLa cells but is resisted by FHL124 cells.

From: Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells

Fig. 2

A–D Cell nuclear morphology imaged via Hoechst 33342 stain showed no remarkable changes in FHL124 cells 24 h after TNFα and CHX stimulation. E–H Nuclear chromatin packaging into apoptotic bodies manifested as condensed nuclei with super bright appearance were seen in HeLa cells 7 h after treatment compared to no treated cells. J Active apoptotic cells measured by flow cytometry probed by FITC-Annexin V and propidium iodide (PI) demonstrated significantly increased apoptosis in HeLa cells 7 h after TNFα and CHX stimulation. On the contrary, less than 20% active apoptotic cells were detected 24 h after TNFα and CHX treatment in FHL124 cells. The Student’s t test was used to compare treated and no treated cells in each cell type, and only p < 0.05 was considered significant. *<0.05, **<0.01, ***<0.001, ****<0.0001.

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