Fig. 5: A mild cFLIP knocking down of FHL124 cells manifested with an increased apoptotic phenotype. | Cell Death & Disease

Fig. 5: A mild cFLIP knocking down of FHL124 cells manifested with an increased apoptotic phenotype.

From: Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells

Fig. 5

A,B Scrambled control shRNA FHL124 cells showed normal nuclear morphology 24 h after TNFα and CHX treatment. C,D KD3 cFLIP knockdown FHL124 cells demonstrated scattered apoptotic cells after 7 h of treatment. E,F KD3 cFLIP knockdown FHL124 cells demonstrated a large volume of apoptotic cells after 24 h of treatment. G,H Active apoptotic cells were analyzed by APC-Annexin V and 7-AAD stain and flow cytometry in scrambled shRNA FHL124 control cells treated with or without 24 h TNFα and CHX stimulation. J,K Active apoptotic cells were analyzed by APC-Annexin V and 7-AAD stain and flow cytometry in KD3 cFLIP knockdown FHL124 cells treated with or without 24 h TNFα and CHX stimulation. L Quantitative data of the ratio of apoptosis in control and cFLIP knockdown FHL124 cells. One-way ANOVA with Tukey’s Honest post-hoc analysis was used to compare between groups, and only p < 0.05 is considered significant. *<0.05, **<0.01, ***<0.001, ****<0.0001.

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