Fig. 4: Damage to parenchyma involves sustained activation of Wnt/β-catenin and TGF-β signaling. | Cell Death & Disease

Fig. 4: Damage to parenchyma involves sustained activation of Wnt/β-catenin and TGF-β signaling.

From: The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease

Fig. 4

A qRT-PCR analysis of Wnt1 and Wnt4 genes relative to the TBP housekeeping gene. B Immunoblot analysis of β-catenin and DVL2 with GAPDH as a loading control. C Immunostaining of β-catenin (green) with DBA marker for collecting ducts (red). DNA is stained by Hoechst (blue). Arrows indicate nuclear staining. Scale bar: 50 μm. D Immunoblot analysis of pSMAD2/3 and total SMAD2/3 with GAPDH as a loading control. E qRT-PCR analysis of genes relative to TBP. Data presented as means ± SEM and significance determined using unpaired two-tailed Student’s t test, n = 3–4. *P < 0.05, **P < 0.01.

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