Fig. 6: Nedd4-2 KO CCD cells display EMT characteristics. | Cell Death & Disease

Fig. 6: Nedd4-2 KO CCD cells display EMT characteristics.

From: The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease

Fig. 6

A Light microscopy of wild type and Nedd4-2 KO cells, scale bar: 200 μm (with higher magnification inset) or immunostaining of vimentin (green) and Phalloidin (red) with Hoechst staining of DNA (blue). Scale bar: 50 μm. B Number of vimentin-positive cells per field of view, n = 4 clones, each with three fields of view. C Light microscopy of cells showing migration distance after scratch, scale bar: 200 μm. D Migration distance quantitated in four clones, n = 3 replicates each. E Immunoblot analysis of N-cadherin with GAPDH as a loading control, untreated or after 72 h 2.5 ng/ml TGF-β1 stimulation. Numbers indicate quantitation of N-cadherin bands relative to untreated wild-type lane, normalized to GAPDH. F Immunoblot analysis of E-cadherin with β-actin as a loading control, untreated or after 3 or 6 days of TGF-β1 stimulation. Numbers indicate quantitation of E-cadherin bands relative to untreated wild type lane, normalized to β-actin. G Immunostaining of E-cadherin (green) after 0, 3, and 6 days of TGF-β1 treatment. DNA is stained by Hoechst (blue). Scale bar: 50 μm. Data presented as means ± SEM (B, D) and significance determined using unpaired two-tailed Student’s t test. ****P < 0.0001.

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