Fig. 7: Low dietary Na+ ameliorates Wnt/β-catenin/TGF-β signaling and kidney disease in Nedd4-2Ksp1.3 mice. | Cell Death & Disease

Fig. 7: Low dietary Na+ ameliorates Wnt/β-catenin/TGF-β signaling and kidney disease in Nedd4-2Ksp1.3 mice.

From: The ubiquitin ligase NEDD4-2/NEDD4L regulates both sodium homeostasis and fibrotic signaling to prevent end-stage renal disease

Fig. 7

Mice were fed a low Na+ (0.05%) diet for 17 days. A Immunoblot analysis of DVL2, β-catenin, pSMAD2/3, and total SMAD2/3 with GAPDH as loading controls. B Immunostaining of αSMA (green) with E-cadherin (red), β-catenin (green) with DBA (red), and vimentin (green) with DBA (red). DNA is stained by Hoechst (blue). Scale bar: 50 μm. C qRT-PCR analysis of genes relative to TBP. D Water intake and urine output over 24 h in metabolic cages. E Haematoxylin and Eosin staining of kidneys, scale bar: 100 μm, 1 mm for inset. F GFR calculated from 24 h urine and blood collection, and serum creatinine and urine levels. Data presented as means ± SEM and significance determined using unpaired two-tailed Student’s t test, n = 3-4. *P < 0.05.

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