Fig. 2: EphA2 protein is enriched in exosomes derived from drug-resistant cells. | Cell Death & Disease

Fig. 2: EphA2 protein is enriched in exosomes derived from drug-resistant cells.

From: Drug-resistant cancer cell-derived exosomal EphA2 promotes breast cancer metastasis via the EphA2-Ephrin A1 reverse signaling

Fig. 2

a Heat map of exosomal proteins differentially expressed in MDA-MB-468 and MDA-MB-468/EPR cells. Exosomes were isolated from drug-resistant breast cancer cells and their parental cells, and then a TMT-based quantitative proteomic method was performed to identify differentially expressed proteins in two kinds of exosomes. b Volcano map of exosomal proteins differentially expressed in MDA-MB-468 and MDA-MB-468/EPR cells. Blue and red dots represent the proteins significantly upregulated in exosomes from MDA-MB-468 and MDA-MB-468/EPR cells. c The Venn diagram of different exosomal proteins in MDA-MB-468 cells and MDA-MB-468/EPR cells. d Fifteen proteins were identified by using a strict cutoff (fold change >2.00, score >100). e The expression of EphA2, ABCB1 (encode P-glycoprotein) in exosomes, and cell lysates were analyzed by using Western blotting; β-actin was used as the loading control. f The expression of EphA2 in two drug-resistant breast cancer cells and their parental cells was studied by using immunofluorescence staining. Scale bars: 10 μm.

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