Fig. 5: Lnc408 suppresses the expression of CBY1 to regulate BCSC self-renewal and stemness maintenance. | Cell Death & Disease

Fig. 5: Lnc408 suppresses the expression of CBY1 to regulate BCSC self-renewal and stemness maintenance.

From: A novel Lnc408 maintains breast cancer stem cell stemness by recruiting SP3 to suppress CBY1 transcription and increasing nuclear β-catenin levels

Fig. 5

A Ectopic CBY1 was transfected into BC cells to check the mammosphere-forming capacity of CSCs, and representative pictures of mammospheres are shown. The right panel represents the statistical results of mammosphere numbers as means ± SD (*P < 0.05; scale bar, 100 μm). B Breast cancer stemness markers (SOX2, Nanog, and CD44 were assessed in CBY1-overexpressed BCSCs by western blotting. C The non-CSC-like BC cells were transfected with control vector, lnc408 or lnc408 combined with CBY1, the mammosphere formation capacities were assessed in serum-free suspended culture. The right panel represents the statistical results of mammosphere numbers as means ± SD (**P < 0.01; ND none detected; scale bar, 100 μm). D, E The mRNA and protein levels of CSC markers (SOX2, Nanog, and CD44) were determined by qRT-PCR (D) and western blotting (E), respectively (*P < 0.05, **P < 0.01). F Pearson correlation analysis of CBY1 and CD44, CBY1 and SOX2 in 474 breast cancer tissues based on Metabric database. G Kaplan–Meier survival analysis to show the BC patients with low CBY1 expression (N = 3851) had poor prognosis, using TCGA database.

Back to article page