Fig. 5: SDPR interacted with and activated ASK1. | Cell Death & Disease

Fig. 5: SDPR interacted with and activated ASK1.

From: Serum deprivation-response protein induces apoptosis in hepatocellular carcinoma through ASK1-JNK/p38 MAPK pathways

Fig. 5

A SDPR-binding proteins were presented by immunoblotting and silver staining. B, C Endogenous co-IP assay and exogenous co-IP assays were conducted to find the interaction of SDPR and ASK1. D Immunofluorescence analysis showed SDPR and ASK1 co-located after co-transfected with FLAG-SDPR and HA-ASK1 in HEK-293T, HCCLM3, and Huh-7 cells. E HA-tagged full-length and three truncated mutants ((1) N-terminal, HA-ASK11-678, (2) Kinase, HA-ASK1679-936, and (3) C-terminal, HA-ASK1937–1374) of ASK1 were cotransfected with FLAG-SDPR in HEK 293T cells and then lysates were immunoprecipitated by utilizing anti-HA antibody. F HA/FLAG-tagged ASK1 N-terminal and MYC-tagged SDPR were cotransfected into HEK 293T and immunoprecipitated using anti-HA/FLAG antibodies to detect ASK1 N-terminus dimerization. *p < 0.05; ***p < 0.01; ***p < 0.001.

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