Fig. 1: USP7 stabilizes BCR-ABL protein in CML cells.
From: Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis
A, B HEK293T cells were transfected with individual deubiquitinases for 48 h, followed by WB assay to measure BCR-ABL at the protein levels. 1. USP7; 2. USP9X; 3. USP14; 4. USP8; 5. UCHL1; 6. USP1; 7. USP15; 8. USP13; 9. UCHL5; 10. USP5; 11. USP36; 12. USP25; 13. BAP1; 14. USP11; 15. USP10; 16. USP30; 17. UCHL3; 18. USP29; 19. USP38; 20. USP39; 21. USPL1; 22. USP49; 23. USP28; 24. CYLD; 25. OTUD4; 26. USP52; 27. USP46; 28. USP53; 29. DUB3; 30. USP44; 31. USP48; 32. USP42; 33. USP50; 34. ATXN3; 35. JOSD2; 36. USP45; 37. OTUD5; 38. USP26; 39. OTUB1; 40. USP3; 41. JOSD1; 42. OTUD6B; 43. USP32P2; 44. OTUB2; 45. JOD1; 46. JOSD3; 47. A2O; 48. PARP11; and 49. CTR. C HEK293T cells were transfected with BCR-ABL and USP7 plasmids as indicated for 48 h, followed cell lysate preparation and WB assays. D HEK293T cells were co-transfected BCR-ABL and USP7 or vector control for increasing periods before being harvested for WB.
