Fig. 2: USP7 upregulates BCR-ABL in CML cells at the protein level.
From: Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis
A K562 and KBM5 cells were infected with lentiviral USP7 for 96 h before being collected for WB and RT-PCR assays. B K562 and KBM5 cells were transfected with siUSP7 for 96 h, followed by WB and RT-PCR assays. C K562 cells were transfected with USP7 or scrambled siRNA for 96 h followed by CHX treatment for indicated periods, cell lysates were subjected to WB analysis. D The statistic analysis of CHX chase assay on BCR-ABL/GAPDH in C. E The BCR-ABL plasmid was co-transfected with USP7 or USP25 plasmids into HEK293T cells, 24 h later, cells were collected for WB against indicated proteins. F siUSP7 was transfected into K562 cells for 96 h, followed by WB against indicated proteins. Scr scramble.
