Fig. 4: USP7 deubiquitinates BCR-ABL in CML cells.
From: Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis
A HEK293T cells were transfected with BCR-ABL and/or USP7 plasmids for 48 h. Cell lysates were then prepared for IP/WB assays as indicated. B K562 and KBM5 were infected with lentiviral USP7 for 96 h, followed by cell lysate preparation and IP/WB assays as indicated. C CML cell lines were infected with sgUSP7 for 96 h, followed by cell lysate preparation and IP/WB assays as indicated. D USP7 was knocked out by the specific siRNA and or USP7 plasmids. Forty-eight hours later, cell lysates were then prepared for IP/WB assays as indicated. E HEK293T cells were transfected with BCR-ABL and WT or C223S USP7 plasmids. Twenty-four hours later, cell lysates were prepared for an IP/WB assay as indicated.
