Fig. 1: Knockdown of B7-H3 promotes DOX-induced senescence. | Cell Death & Disease

Fig. 1: Knockdown of B7-H3 promotes DOX-induced senescence.

From: B7-H3 suppresses doxorubicin-induced senescence-like growth arrest in colorectal cancer through the AKT/TM4SF1/SIRT1 pathway

Fig. 1

A Western blot analysis of B7-H3 in CRC stable cell lines with B7-H3 inhibition (shB7-H3) or their control cell lines (sh-NC). β-Actin served as a loading control. B Western blot analysis of p21 and p53 in sh-NC cells or shB7-H3 CRC cells treated with DOX. β-Actin served as a loading control. C SA-β-Gal activity of sh-NC cells or shB7-H3 CRC cells treated with DOX was examined. Scale bar, 100 μm. One representative image from three reproducible experiments is shown. The percentages of SA-β-gal-positive cells are shown in the bar graph. D, E SAHF activity of sh-NC cells or shB7-H3 HCT116 cells and RKO cells with DOX treatment was examined. Scale bar, 50 μm. One representative image from three reproducible experiments is shown. The percentages of SAHF-positive cells are shown in the bar graph. f Cell viability in sh-NC cells or shB7-H3 CRC cells treated with DOX after 24, 36, 48, and 96 h was examined by CCK-8 assays. G The colony formation of sh-NC cells or shB7-H3 CRC cells treated with DOX was examined. One representative image from three reproducible experiments is shown. The number of colonies is shown in the bar graph. H Cell cycle analysis by PI staining in sh-NC cells or shB7-H3 CRC cells with DOX treatment was examined through flow cytometry. The data represent the means ± SEM. *P < 0.05; ***P < 0.001.

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