Fig. 1: Single-cell profiling revealed cell heterogeneity in mice bladder mucosa layer under healthy and injury conditions. | Cell Death & Disease

Fig. 1: Single-cell profiling revealed cell heterogeneity in mice bladder mucosa layer under healthy and injury conditions.

From: Single-cell analysis reveals urothelial cell heterogeneity and regenerative cues following cyclophosphamide-induced bladder injury

Fig. 1

A Overview of the experimental workflow. B t-Distributed stochastic neighbor embedding (t-SNE) visualization of all cells from healthy, acute injury and chronic injury conditions. Cells are colored by clusters. C t-SNE visualization annotated by injury conditions. D The proportion of each cell type under each condition. E t-SNE visualization of cells from each condition colored by cycling score. F Percentages of cycling cells in urothelium for each condition. G Fluorescent immunostainings from the indicated conditions show nuclei (DAPI) in blue, E-cad in green, and Ki67 in red (scale bar 50 microns). H Bar plot shows the percentage of Ki67 positive cells in urothelial cells (n = 12 per condition, mean with SD, t-test, ****p < 0.0001).

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