Fig. 5: Cell diversity within the mesenchymal lineage. | Cell Death & Disease

Fig. 5: Cell diversity within the mesenchymal lineage.

From: Single-cell analysis reveals urothelial cell heterogeneity and regenerative cues following cyclophosphamide-induced bladder injury

Fig. 5

A UMAP visualization of subclustered mesenchymal cells in three conditions. B Dot plot shows expression of signature genes for fibroblast, myofibroblast and cycling subset in each cluster. The color represents scaled average expression of marker genes in each cluster, and the size indicates the proportion of cells expressing marker genes. C Heatmap of SCENIC binary regulon activities shows the distinct regulon activity between myofibroblast and fibroblast. D Binary activity of representative regulons for fibroblast and myofibroblast subset projected on UMAP. E Immunostainings of a-SMA (in red) and Cd34 (in green) on section of healthy bladder show the existence of myofibroblast and fibroblast, and their location (scale bar 50 microns). F RNA velocity of mesenchymal cells under each condition. Clusters are labeled with same color code as in A. G Bar plot show the proportion of fibroblast cells which can transform to myofibroblasts. H Violin plots show the ECM-score in each cluster under each condition. Condition are labeled with same color code as in G.

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