Fig. 5: TP53INP1 Drosophila homolog dDOR activates autophagy and mitophagy.
From: TP53INP1 exerts neuroprotection under ageing and Parkinson’s disease-related stress condition
A Confocal images of living third instar larvae fat bodies from control (w1118) and dDOR homozygous null mutant (−/−) stained with Lysotracker. B Quantification of lysosome density (lysotracker positive puncta per 100 µm2) (left panel) and quantification of average lysosomal size (µm ± SD) in control and dDOR homozygous mutant fat bodies. Three samples analyzed for each condition. Mann–Whitney U test result: ***P < 0.001. C Confocal images of living fat bodies from CG-GAL4 UAS-mCherry::Atg8a UAS-dDOR::GFP third instar larvae. D Confocal images of elav-GAL4 UAS-Atg8a::GFP living adult fly brains (10-day old females at 29 °C) stained with lysotracker and expressing or not a UAS-dDOR-F transgene. E Quantification of Atg8a::GFP positive vesicles (puncta per 100 µm2 ± SEM) in brains of elav-GAL4 UAS-Atga::GFP adult flies expressing or not a UAS-dDOR transgenes (dDOR-F but not dDOR-L contains EcR binding motif). Number of analyzed samples: 20 for elav-GAL4 control, 14 for elav-GAL4 UAS-dDOR-F, 8 for elav-GAL4 UAS-dDOR-L. Mann–Whitney U test results: ***P < 0.001, **P < 0.001. F Proportion of Atg8::GFP puncta co-stained with Lysotracker (% ±SEM) and proportion of Lysotracker positive puncta co-stained with Atg8a::GFP in flies expressing or not UAS-dDOR transgenes. Number of analyzed samples: 20 for elav-GAL4 control, 14 for elav-GAL4 UAS-dDOR-F, 8 for elav-GAL4 UAS-dDOR-L. Mann–Whitney U test results: **P < 0.01, *P < 0.05. G Confocal images of elav-GAL4 UAS-mtKeima living adult brains (7-day old females at 29 °C) from control, dDOR homozygous mutant (−/−) or UAS-dDOR transgenic flies. Left panels show a superposition of mtKeima signals captured upon 458 nm (green chanel) and 561 nm (red channel) excitation. Central panels show mtKeima signal upon 561 nm excitation only. Right panels show ratiometric images obtained by dividing mtKeima signal intensities upon excitation at 561 nm and 458 nm. H Quantification of mitophagy index (±SEM) measured in elav-GAL4 UAS-mtKeima living adult brains from control, dDOR homozygous mutant or UAS-dDOR-F and dDOR-F transgenic flies. Mitophagy index corresponds to total pixel intensity of ratiometric images (mtKeima signal at 561 nm/signal at 458 nm) normalized by total mitochondrial area. Number of analyzed samples: 15 for elav-GAL4 control, 9 for elav-GAL4 dDOR−/− 9 for elav-GAL4 UAS-dDOR-F, 7 for elav-GAL4 UAS-dDOR-L. Mann–Whitney U test result: **P < 0.01.
