Fig. 6: MM-MΦs-conferred myeloma chemoresistance requires BMI1 overexpression.

A The apoptotic rate of 5TMG1 cells treated with indicated drugs alone or with wt MФs or BMI1-KO MФs for 24 h. Statistical significance was determined by two-tailed Student t-test between drug treated 5TGM1 cells cultured alone and with wt MM-MФs, or between drug treated 5TGM1 cells cultured with wt MM-MФs and with BMI1-KO MM-MФs, *P < 0.05. B Western blot showed the activation status of apoptotic protein PARP and Capase-3 of 5TGM1 cells treated with indicated drugs alone or with wt or BMI1-KO MФs for 24 h. C Western blot showed the protein expression or phosphorylation changes of 5TGM1 cells cultured alone or with wt or BMI1-KO MФs. D Pathway enrichment of differential regulated genes from RNA-seq data of wt vs BMI1-KO MM-MФs, comparing with GO cellular component genesets. E RT-qPCR showed the mRNA expression of Galectin-3 was decreased in BMI1-KO MM-MФs, relative to wt MM-MΦs (left column). Western blot showed the protein level of Galectin-3 was lower in BMI1-KO MM-MФs (right panel). Statistical significance was determined by two-tailed Student t-test between wt and BMI1-KO MM-MФs, **P < 0.01.