Fig. 2: p27 localizes in the cytoplasm during prolonged glucose starvation. | Cell Death & Disease

Fig. 2: p27 localizes in the cytoplasm during prolonged glucose starvation.

From: p27 controls autophagic vesicle trafficking in glucose-deprived cells via the regulation of ATAT1-mediated microtubule acetylation

Fig. 2

A Immunoblot for P-T172-AMPK and AMPK of p27+/+ and p27−/− MEFs in full medium (0 h) or glucose-starved for 48 h. B p27 immunoblot of p27+/+ and p27−/− MEFs in full medium (0 h) or glucose-starved for 48 h. β-actin was used as loading control. C Graph shows mean ± SEM of quantification of p27 levels from experiments shown in B, expressed as fold change from full medium condition from n = 5 independent experiments. D p27+/+ and p27−/− MEFs were glucose-deprived for the indicated times and immunostained for p27. Nuclear DNA was stained with Hoechst 33342. Graphs display the fluorescence intensity (arbitrary unit) in each channel over the distance depicted by the arrows. E Graph shows means ± SEM of the cytoplasm to nuclear ratio of p27 fluorescence intensity in p27+/+ and p27−/− MEFs in full medium (0 h) or glucose-starved for 48 h from n = 3 independent experiments. At least 64 cells were analyzed per condition per experiment. C, E Statistical significance was evaluated by two-tailed Student’s t test with Welch’s correction.

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