Fig. 5: Comparison of miR-125a-5p and miR-200b-3p isomiR expression in benign nevi (BN), cutaneous melanoma (CM), and multiple primary melanoma (MPM).
From: Unraveling the role of microRNA/isomiR network in multiple primary melanoma pathogenesis
a Floating bar chart of miR-125a-5p isomiR expression. Canonical miR-125a-5p (hsa-miR-125a-5p|0|0) shows a lower expression level and opposite expression trend in BN, CM, and MPM if compared to its shorter isomiR (hsa-miR-125a-5p|0|−2). Bars represent min–max and median values. b Floating bar chart of miR-200b-3p isomiR expression showing the same expression trend for canonical miR-200b-3p (hsa-miR-200b-3p|0|0) and its longer isomiR (hsa-miR-200b-3p|0|+1) in MPM groups. Canonical miR-200b-3p was not detected in CM samples. Bars represent min–max and median values. c Box and whiskers graph representation of canonical miR-125a-5p expression assessed with miRCURY LNA assay and overall expression of all miR-125a-5p isoforms, detected using miSCRIPT assay. Results show that the combined expression of miR-125a-5p isoforms of levels is higher in MPM compared to CM. The bar shows minimum and maximum values; superimposed points represent all individual values. d Before–after plot of canonical miR-125a-5p expression (miRCURY LNA assay) and all miR-125a-5p isoforms (miSCRIPT assay) show an opposite trend in the first and second melanoma from the same patient. e Box and whiskers graph representation of canonical miR-200b-3p expression assessed with miRCURY LNA assay and overall expression of all miR-200b-3p isoforms, detected using miSCRIPT assay. Results show that in both there is a higher expression in MPM compared to CM. The bar shows minimum and maximum values; superimposed points represent all individual values. f Before–after plot of canonical miR-200b-3p expression (miRCURY LNA assay) and all miR-200b-3p isoforms (miSCRIPT assay) show both a higher expression in the second melanoma compared to the first and from the same patient.
