Fig. 3: miR-135b promotes cell migration, invasion, anti-apoptosis, and angiogenesis in NSCLC.

A, B Representative pictures of cell migration (A) and invasion (B) in H292 and A549cells analyzed by transwell assay. Scale bars, 100 μm. C Representative pictures of cell apoptosis after treatment with cisplatin for 24 h analyzed by TUNEL staining (green). Cells were counterstained with DAPI. Scale bars, 20 μm. D Cell migration was assessed by culturing HUVECs with conditioned medium collected from the indicated NSCLC cells. Scale bars, 100 μm. E Representative images of tubule formation by HUVECs cultured in Matrigel-coated plates with conditioned medium collected from the indicated NSCLC cells. Scale bars, 50 μm.