Fig. 8: Immunofluorescence analysis of 3D brain organoids at day 70.
From: Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs
A Cellular identity characterization in FMRP-WT (top) and FMRP-KO (bottom) brain organoids. Immunostaining at day 70 revealed the presence of cells positive for pan-neuronal marker MAP2 (red) and GFAP expressing astrocytes (green). Nuclei were stained with DAPI (blue). Scale bar: 500 μm. B Higher magnification of representative GFAP-positive regions within FMRP-WT (top) and FMRP-KO (bottom) brain organoids at day 70. Scale bar: 20 μm. C Bar charts showing the fluorescence intensity quantification of MAP2 signal (p = 0.84, MW test) and GFAP signals (**p < 0.01; WT n = 42/7/2, KO n = 29/6/2, slices/organoids/batches). D Scatter plot of astrocyte density in FMRP-WT (n = 49/18/8/2, FOVs/slices/organoids/batches) and FMRP-KO brain organoids (n = 45/11/5/2, FOVs/slices/organoids/batches) at day 70 (**p < 0.01, MW test).
