Fig. 1: DRD5 receptor is highly expressed in colonic macrophages.
From: DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization
A RT-qPCR analysis of gene expression of DRD1, DRD2, DRD3, DRD4, and DRD5 in IECs (CD45-Epcam+) and LP cells (CD45+Epcam-) isolated from WT mice (n = 3 mice per group). B RT-qPCR analysis of gene expression of DRD1, DRD4, and DRD5 in T cells, B cells, NK cells, DC cells, monocytes, neutrophils, macrophages, and ILCs cells isolated from the colonic LP of WT mice (n = 3 mice per group). C Immunofluorescent labeling of TH (green), Cx3cr1(red), and DAPI (blue) in colon sections from Cx3cr1 reporter mice. The close proximity of macrophages with dopaminergic neurons is indicated by arrow. Scale bar, 30 µm. D Immunofluorescent labeling of DRD5 (green), F4/80 (red), and DAPI (blue) in colon sections from WT mice. The merging of DRD5 with F4/80+ in macrophages indicated by the arrowhead. Scale bar, 30 µm. E Immunofluorescent labeling of DRD5 (green), Arg1(red), and DAPI (blue) in colon sections from WT mice. The merging of DRD5 with Arg1+ in macrophages indicated by the arrowhead. Scale bar, 30 µm. F Immunofluorescent labeling of DRD5 (green), Inos (red), and DAPI (blue) in colon sections from WT mice. The merging of DRD5 with Inos+ in macrophages indicated by the arrowhead. Scale bar, 30 µm. Data are pooled from three independent experiments (A, B). Error bars show means ± SEM. ***p < 0.001. Two-tailed unpaired student’s t-test.
