Table 4 Antibodies used in this study.
From: Regulation of sarcomagenesis by the empty spiracles homeobox genes EMX1 and EMX2
Antibody | Company | Dilution | |||
|---|---|---|---|---|---|
WB | IHQ | ||||
DIL | DA | ||||
EMX1 (rabbit polyclonal) | Abcam | ab136102 | 1:1000 | 1:100 | EDTA |
EMX2 (mouse polyclonal) | Abcam | ab171818 | 1:1000 | - | - |
EMX2 (rabbit polyclonal) | Invitrogen | PA5-34415 | - | 1:500 | TC |
α-tubulin (mouse monoclonal) | Sigma-Merck | T9026 | 1:10,000 | - | - |
KI67 (rabbit monoclonal) | MAD | 000310-QD | - | 1:250 | TC |
NESTIN (rat polyclonal) | SCB | SC-101541 | - | 1:100 | TC |
Rabbit anti-mouse HRP (polyclonal)a | Abcam | ab97046 | 1:5000 | - | - |
Goat anti-rabbit HRP (polyclonal)a | Abcam | ab97051 | 1:5000 | - | - |
Goat anti-rabbit HRP (polyclonal)a | JAC | 111-035-003 | - | 1:400 | - |
Goat anti-rat HRP (polyclonal)a | JAC | 112-035-003 | - | 1:400 | - |
Rabbit anti-goat HRP (polyclonal)a | Abcam | ab97100 | - | 1:400 | - |
