Fig. 3: Inhibition of FEN1 by NO.0449-0145 leads to the accumulation of DNA damage. | Cell Death & Disease

Fig. 3: Inhibition of FEN1 by NO.0449-0145 leads to the accumulation of DNA damage.

From: Small-molecule inhibition of APE1 induces apoptosis, pyroptosis, and necroptosis in non-small cell lung cancer

Fig. 3

a Western blot assay to determine γ-H2AX levels in A549 and NCI-H460 cells following different dose of NO.0449-0145 treatments. b, c Immunofluorescence staining of γ-H2AX foci and 53BP1 foci in cells. The quantification of the foci numbers per cells were showed in right panel. d Comet assay of A549 and NCI-H460 cells treated with or without NO.0449-0145. Representative images for the quantification of the average tail moment reported for each treatment condition. Each experiment was repeated three times. *p < 0.05; **p < 0.01; ***p < 0.001.

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