Fig. 4: NO.0449-0145 induces cell death in NCI-H460 and A549 cells. | Cell Death & Disease

Fig. 4: NO.0449-0145 induces cell death in NCI-H460 and A549 cells.

From: Small-molecule inhibition of APE1 induces apoptosis, pyroptosis, and necroptosis in non-small cell lung cancer

Fig. 4

a Morphological analysis of NCI-H460 and A549 cells with different dose of NO.0449-0145 treatment for 24 h. The red arrows indicate the apoptotic bodies; the black arrows point to bubbling of cells. b Annexin V/PI staining and flow cytometry analysis of NCI-H460 and A549 cells with different dose of NO.0449-0145 treatment for 24 h. c Levels of BAD, BAK, Mcl-1 and Bcl-XL assessed by western blotting for the cells treated with NO.0449-0145 for 12 h. d Representative Hoechst/PI fluorescence images of cells with different treatment. e Western blotting analysis of basic RIPK3, MLKL, phosphorylation of RIPK3 (P-RIPK3), and MLKL (P-MLKL) in A549 and NCI-H460 cells. f Western blotting analysis of caspase-4 and GSDMD in A549 and NCI-H460 cells. g Secreted IL-1β from cells treated with NO.0449-0145 for 24 h. Each experiment was repeated three times. The significance between treated samples and control samples was determined using a t-test. *p < 0.05; **p < 0.01; ***p < 0.001.

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