Fig. 1: FOXM1 levels modulate cell fate profile in response to antimitotics.
From: FOXM1 repression increases mitotic death upon antimitotic chemotherapy through BMF upregulation
a Representative time-lapse sequences of slippage/mitotic exit (in gray) and death in mitosis (DiM, in red) cell fates of HDFs treated with antimitotics. Time in h:min, from nuclear envelope breakdown (NEB) to each cellular outcome. Scale bar, 20 µm. b FOXM1 transcript levels in 87-year-old HDFs transduced with control (empty vector) and FOXM1-overexpressing lentiviruses. c FOXM1 transcript levels in mock- and FOXM1 siRNA-depleted 10-year-old HDFs. d Individual cell fate profiling (exit vs. DiM) of control (empty) (n = 200) or FOXM1-overexpressing 87 y HDFs (n = 200) treated with 5 µM STLC. MD, mitotic duration. e Cell fate percentage of the experiments described in d. f Mitotic duration of the individual cell fates shown in d. g Individual cell fate profiling (exit vs. DiM) of mock- (n = 182) and siFOXM1-depleted (n = 172) 10 y HDFs treated with 5 µM STLC. MD, mitotic duration. h Cell fate percentage of the experiments described in g. i Mitotic duration of the individual cell fates shown in g. Data information: In b, c data are mean ± S.D. from n = 3 independent experiments; *p ≤ 0.05, **p ≤ 0.01 (two-tailed paired t test). In e, h data are mean ± S.D. from n = 3 independent experiments; ****p ≤ 0.0001 (two-tailed χ2). In f, i values are mean. *p ≤ 0.05, ****p ≤ 0.0001 (two-tailed Mann–Whitney test).
