Fig. 5: Arzanol causes damage-induced fragmentation of mitochondria during starvation. | Cell Death & Disease

Fig. 5: Arzanol causes damage-induced fragmentation of mitochondria during starvation.

From: High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol

Fig. 5

A Shown are microscopy images of mito-DsRed-expressing HeLa cells immunofluorescently labeled for endogenous TOM20. During starvation, arzanol induces fragmentation of both inner (mito-DsRed) and outer (TOM20) mitochondrial membranes. Cells were starved in serum- and amino acid-free medium for 2 h while incubated with 5 µM arzanol. Scale bars in all panels are 20 µm. B Immunoblot for markers of mitochondrial damage (OPA1 cleavage, Parkin ubiquitination (Ub), PINK accumulation) reveals that arzanol-induced mitochondrial fragmentation is independent of apoptosis induction. HeLa cells stably expressing mito-DsRed GFP-Parkin were incubated with serum-free full medium or starvation medium and treated with 5 µM arzanol or a combination of 4 µM antimycin and 10 µM oligomycin (A/O) with/without 10 µM QVD for 6 h. Shown blots are representative of three independent experiments. L and S indicate long and short forms of OPA1, respectively. Asterisk indicates an unspecific band.

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