Fig. 1: The expression pattern of HDAC6 in the neonatal mouse ovaries.
From: HDAC6 regulates primordial follicle activation through mTOR signaling pathway
A Cellular localization of HDAC6 in newborn ovaries. Newborn mice ovaries were stained for HDAC6 (green) and the oocyte specific marker DDX4 (red) at the indicated time points. The nuclei were counter-stained by DAPI (blue). HDAC6 was mainly localized to the cytoplasm of both oocytes and granulosa cells in either primordial follicles or growing follicles. B qRT-PCR assay showed that the expression level of Hdac6 mRNA was increased from 1 dpp to 5 dpp and decreased on 7 dpp. C Western blotting assay showed that the level of HDAC6 protein was increased from 1 dpp to 5 dpp and decreased on 7 dpp as well. The experiments were repeated at least three times. And representative images are shown. The data are presented as the means ± SEM. Scale bars: 50 μm.
