Fig. 3: Identification of Erbb2 positive mammary tumors by differentially expressed genes (DEGs).
From: Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway
A Pie chart representation of KEGG pathway enrichment of DEGs between ++Oxtr tumors to WT mammary gland. B–E Gene set enrichment analysis (GSEA) showed upregulated genes of cancer proliferation cluster (B), breast cancer-related pathways (C, D), and ERBB2 breast cancer-related pathways (E) were significantly enriched in ++Oxtr tumors. Significance was determined by normalized enrichment score (NES) and FDR. F Heatmap representation of gene expression patterns in human HER2+ breast cancer patients to normal breast3 (Data from GSE61) and ++Oxtr tumors to WT mammary gland. G Validation of RNAseq results by qPCR. Gene expression of Erbb2, Grb7, Esr1, and Pgr, n = 6. H Gene expression of Tgfa, Egfr, Akt1, and Brca1, n = 6. I Gene expression of Tgfβ1, Pten, p53, and Bcl2, n = 6. J Gene expression of Prlr, Csn2, and Wap, n = 6. Data were represented as mean ± SD. **P < 0.01, ***P < 0.001, calculated using two-tailed unpaired t-test.
