Fig. 1: Nprl3 and Nprl2 expression levels are interdependent.
From: FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila
A Western blot analysis of Nprl3 protein in the indicated dsRNA treated S2 cells. B Western blot analysis of Nprl3 protein in the ovaries of MTD > mCherry RNAi (control), MTD > nprl2 RNAi, MTD > nprl3 RNAi and MTD > Tsc1 RNAi flies. C Western blot analysis of Nprl3 protein in the ovaries of yw (control), nprl21 and nprl31. D Western blot analysis of Nprl3 protein in the ovaries of yw, TorA948V/+ and TorA948V/+; iml11. E Western blot analysis of HA-Nprl3 protein in the ovaries of MTD > HA-Nprl3 and MTD > HA-Nprl3, nprl2 RNAi flies. F Western blot analysis of the HA-Nprl2 protein in the ovaries of MTD > HA-Nprl2 and MTD > HA-Nprl2, nprl3 RNAi flies. G Western blot analysis of Nprl3 protein in the ovaries of nos > mCherry RNAi (control), nos > nprl2 RNAi, nos > nprl2 RNAi, huwe1 RNAi and nos > huwe1 RNAi flies. α-Tubulin was used as a loading control. Similar western blot results were observed in more than three independent experiments for each group.
