Fig. 4: FKBP39 associates with Nprl2 and decreases its expression.
From: FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila
A S2 cells were transfected with the indicated construct inserted with HA-tagged Nprl2 coding region. The cells were starved for the indicated time and the HA-Nprl2 levels were measured by western blot. α-Tubulin was used as a loading control for western blot. B S2 cells were co-transfected with HA-tagged Nprl2 and V5-tagged FKBP39 or lacZ (control) plasmids. Cells were lysed and immunoprecipitated using beads coated with anti-HA antibody. Cell lysates (inputs) and immunoprecipitates (IP) were detected by western blot. C S2 cells were co-transfected with the GFP (control) or fkbp39 dsRNA, constructs expressed HA-tagged Nprl2 and V5-tagged lacZ. The levels of HA-Nprl2 and V5-lacZ were detected by western blot. V5-lacZ was used as a loading control for western blot. Similar western blot results were observed in more than three independent experiments for each group.
