Fig. 6: Nutrient signal and USPD independently controls Nprl3 stability.
From: FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila
A S2 cells were transfected with GFP (control) or nprl2 dsRNA. Cells were treated with amino acid starvation for 2 h. The levels of Nprl3 were detected by western blot. B The ovaries from yw (control) and nprl21 were dissected and cultured in amino-acid-free Schneider’s medium (AA−) for 2 h. The levels of Nprl3 were detected by western blot. C. The ovaries from MTD > Nprl3-HA (control) and MTD > Nprl3-HA, nprl2 RNAi were dissected and cultured in amino-acid-free Schneider’s medium (AA−) for 2 h. The levels of HA-Nprl3 were detected by western blot. D S2 cells were transfected with GFP (control) or huwe1 dsRNA. Cells were treated with amino acid starvation for 2 h. The levels of Nprl3 were detected by western blot. E The ovaries from nos > mCherry RNAi (control) and nos > huwe1 RNAi were dissected and cultured in amino-acid-free Schneider’s medium (AA-) for 2 h. The levels of Nprl3 were detected by western blot. F Western blot analysis of Nprl3 protein in the ovaries of Nos > mCherry RNAi (control), Nos > nprl2 RNAi, Nos > nprl2 RNAi, fkbp39 RNAi and Nos > fkbp39 RNAi flies. α-Tubulin was used as a loading control. Similar western blot results were observed in more than three independent experiments for each group.
