Fig. 7: Fat clearance by NCT acts through CYP26A1. | Cell Death & Disease

Fig. 7: Fat clearance by NCT acts through CYP26A1.

From: Liver fat storage is controlled by HNF4α through induction of lipophagy and is reversed by a potent HNF4α agonist

Fig. 7

A RT-PCR analysis of hepatic CYP26A1 mRNA level (Normal chow control; N = 5 and for DMSO and NCT; N = 10). B RT-PCR analysis of CYP26A1 mRNA level in T6PNE cells treated for 2 days with DMSO, NCT (10 μM), RA (10 μM), NCT + RA (10 μM), NFT (20 μM), fenretinide (5 μM), 4-OH-RA (20 μM) on 0.25 mM palmitate and DMSO w/o palmitate. C CYP26A1 is required for fat clearance by NCT. T6PNE cells treated with palmitate (0.25 mM) plus the indicated compounds for 2 days, including the inhibitors ABT (10 mM, broad CYP inhibitor) and Talarozole (10uM, selective CYP26 inhibitor). D Quantification of the effect of CYP inhibitors on fat clearance by NCT (vs NCT for significance). E Metabolites from CYP26-mediated RA metabolism induce fat clearance. Representative images of T6PNE cells treated for 2 days with NCT or the RA metabolites 4-OXO-RA, 5,6-epoxy-RA, or 4-OH-RA (20μM) and stained with Nile Red. F Quantification of effect of RA metabolites on fat clearance. Values represent the mean ± SE of 4 biological replicates, *p < 0.05, **p < 0.01 (vs DMSO). Scale bar = 100 μm.

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