Fig. 3: Characterization of adipocyte death and CLS formation post laser injury. | Cell Death & Disease

Fig. 3: Characterization of adipocyte death and CLS formation post laser injury.

From: Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages

Fig. 3

A Phosphatidylserine externalization detected via Annexin V staining (magenta) 24 h post laser injury. Nucleus of depleted adipocyte shows no DAPI staining, while some surrounding ATMs are DAPI positive (white; highlighted by arrows), validating DAPI staining (N = 3). B Induced CLS formed 48 h post laser injury in AT explants of chow-fed (chow) and HFD-fed (HFD) mice. C Frequency of CLS formation after laser injury (N = 3). Asterisks mark depleted adipocytes, scale bars = 50 µm.

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