Fig. 5: In vivo formed CLS in lean mice show comparable inflammation marker expression to induced CLS.

Whole mount antibody staining of CLS formed in vivo in lean mice under homeostatic conditions. A–C ATMs in in vivo formed CLS express pro-inflammatory markers CD11c, CD86, and CD9, while interstitial ATMs are negative (highlighted by arrows). D, E Interstitial ATMs express the anti-inflammatory markers CD206 and CD301 (highlighted by arrows), while ATMs in CLS are negative. F, G Expression of CD64 (all macrophages) or TREM2 (highlighted by arrows). Asterisks mark adipocytes inside CLS. Scale bars = 100 µm.