Fig. 6: RNA sequencing reveals metabolic activation following adipocyte death. | Cell Death & Disease

Fig. 6: RNA sequencing reveals metabolic activation following adipocyte death.

From: Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages

Fig. 6

A GFP positive cells were sorted in CD11chigh and CD11clow groups for RNA sequencing 48 h following laser injury. B Pathway analysis of gene expression profiles between the sorted groups. C, D Heatmaps comparing differentially expressed genes related to either M1 or M2 polarization (C) or associated with metabolic activation, lipid metabolism or chemokines and their receptors (D). Colors indicate the log2 fold change of gene expression in the 2 individual experiments from average across all samples. Expression of genes shown is significantly different between CD11c high and CD11c low group (p < 0,001). E Expression of markers differentiating between M1 polarization (CD38, CD274) and metabolic activation (CD36) and markers for lysosomal exocytosis in induced CLS. F Expression of the respective markers in vivo in lean mice. Asterisks mark adipocytes within CLS, positive interstitial ATMs are highlighted by arrows. Scale bars = 50 µm.

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