Table 1 The primers in qRT-PCR.

circ_AFF4

Forward: 5′-GCATCGGTTTCTGGTGATGT-3′

Reverse: 5′-CGGTTCATGTTGCTTAGTTG-3′

miR-135a-5p

Forward: 5′-UAUGGCUUUUUAUUCCUAUGUGA-3′

Reverse: 5′-AACGCTTCACGAATTTGCGT-3′

FNDC5

Forward: 5′-GTGGTGAGCTGGGATGTTCT-3′

Reverse: 5′-GCCTGCACGTGGACTATGTA-3′

GAPDH

Forward: 5′-CGACAGCAGCCGCATCTT-3′

Reverse: 5′-CCAATACGACCAAATCCGTTG-3′

U6

Forward: 5′-CTCGCTTCGGCAGCACA-3′

Reverse: 5′-AACGCTTCACGAATTTGCGT-3′

ALP

Forward: 5′-GAACGTGGTCACCTCCATCCT-3′

Reverse: 5′-TCTCGTGGTCACAATGC-3′

BMP4

Forward: 5′-AGCATGTCAGGATTAGCCGA-3′

Reverse: 5′-TGGAGATGGCACTCAGTTCA-3′

RUNX2

Forward: 5′-CGGAATGCCTCTGCTGTTAT-3′

Reverse: 5′-TTCCCGAGGTCCATCTACTG-3′

Spp1

Forward: 5′-CGCAGACCTGACATCCAGT-3′

Reverse: 5′-GGCTGTCCCAATCAGAAGG-3′

Colla1

Forward: 5′-CCTGGATGCCATCAAAGTCT-3′

Reverse: 5′-AATCCATCGGTCATGCTCTC-3′