Fig. 5: Promotion of B10 cell generation by SCFAs is independent of GPCR activity. | Cell Death & Disease

Fig. 5: Promotion of B10 cell generation by SCFAs is independent of GPCR activity.

From: Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function

Fig. 5

A, B The mRNA expression of GPR41, GPR43, and GPR109A detected by RT-qPCR in murine splenic naive B cells (A) and B cells cultured with or without 500 μM SCFAs under the existence of CD40 mAb for 2 days (B). C–F B10 cell frequency in purified splenic B cells cultured with or without 500 μM NaBu, 10 μM GPR43 antagonist (C), 1 μM GPR43 agonist (D), 1 μM GPR41 antagonist AR420626 (E), or 1 mM GPR109A agonist niacin (F) under the existence of CD40 mAb or LPS for 2 days. The data are presented as mean ± SD from three independent experiments. *P < 0.05, **p < 0.01, and ***p < 0.001 compared between the groups as indicated.

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