Fig. 6: Promotion of B10 cell generation by butyrate depends on its HDAC inhibitory activity. | Cell Death & Disease

Fig. 6: Promotion of B10 cell generation by butyrate depends on its HDAC inhibitory activity.

From: Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function

Fig. 6

A Purified splenic B cells were polarized to Bregs after a 2-day coculture with indicated SCFAs or Trichostatin A (TSA, an HDAC inhibitor with high efficiency) in the presence of LPS; the nuclear protein was prepared and tested for HDAC inhibition by ELISA. B–D B10 cell frequency in purified splenic B cells cultured with or without sodium acetate (B), HDAC inhibitors TSA or vorinostat (C), or HAT inhibitor (HATi) anacardic acid (D) under the existence of CD40 mAb or LPS for 2 days. Results were shown as representative FACS plots or bar graphs. The data are presented as mean ± SD from three independent experiments. *P < 0.05, **p < 0.01, and ***p < 0.001 compared to Ctrl or as indicated.

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