Fig. 6: Silencing CTD-2528L19.6 promotes fibroblast activation of MRC-5 cells.
From: LncRNA CTD-2528L19.6 prevents the progression of IPF by alleviating fibroblast activation
A Fluorescence in situ hybridization detects the localization of CTD-2528L19.6 in MRC-5 cells. For clarity, CTD-2528L19.6 was abbreviated as CTD in the figures. 18 S and U6 are the markers of cytoplasm and nuclei, respectively. Blue nuclei are stained with DAPI. Scale bar: 50 μm. B, C The expression of CTD-2528L19.6, Fn1, Collagen 1α1 after silencing CTD-2528L19.6 in MRC-5 cells were detected by qRT-PCR. n = 4. D Western blot was used to detect the expression of Fn1 and Collagen I after SSi-CTD transfection. n = 6. Wound-healing migration assay (E) and EdU proliferation assay (F) were used to assay the effect of SSi-CTD on migration and proliferation in MRC-5 cells. n = 4. Scale bars, 200 μm in E and 50 μm in F. G Immunofluorescence was applied to examine the fibroblasts into myofibroblasts transition after transfecting SSi-CTD. Scale bar, 20 μm. *P < 0.05; **P < 0.01.
