Fig. 7: LncRNA CTD-2528L19.6 inhibits the TGF-β1-induced fibroblast activation in MRC-5 cells.
From: LncRNA CTD-2528L19.6 prevents the progression of IPF by alleviating fibroblast activation
qRT-PCR analysis of the expression of CTD-2528L19.6 (A) and Fn1, Collagen 1α1 (B) in MRC-5 cells after transfection CTD-2528L19.6 under TGF-β1 induced fibroblast activation. n = 4. C Western blot results demonstrate that the overexpression of CTD-2528L19.6 inhibits the pro-fibrotic effect of TGF-β1 in MRC-5. n = 9. Wound-healing assay (D) and EdU proliferation assay (E) reveals the inhibitory effects of CTD-2528L19.6 on TGF-β1-induced migration and proliferation in MRC-5 cells. n = 4. Scale bars, 200 μm in D and 50 μm in E. F Fibroblast to myofibroblast transition was observed by immunofluorescence. Scale bar, 20 μm. *P < 0.05; **P < 0.01.
