Fig. 7: Activation of GPR17 inhibited glioma formation. | Cell Death & Disease

Fig. 7: Activation of GPR17 inhibited glioma formation.

From: G-protein-coupled receptor GPR17 inhibits glioma development by increasing polycomb repressive complex 1-mediated ROS production

Fig. 7

A U87MG and U251 cells treated with vehicle or MDL29951 (300 μM) for 48 h, and CCK-8 assay was performed to examine viable cell numbers. Data represent the means ± SEM from three independent experiments. B–D BALB/c nude mice with subcutaneously xenotransplanted tumor were intraperitoneally injected with 10 mg/kg MDL29951 for 16 days, and then tumor samples were collected for the measurements of tumor sizes (B), volumes (C), and weights (D), (n = 7). E H&E staining of sections from subcutaneously xenotransplanted tumors. Scale bar 10 μm. F, G Immunofluorescent staining against Ki67 (F) and TUNEL (G) of the tumor sections, scale bar 50 μm. H IHC staining against p-PKA or RNF2 of the subcutaneously xenotransplanted tumor. Scale bar 50 μm. I A diagram depicting the working model of the inhibitory effect of GPR17 on glioma tumorigenesis. For all panels, *p < 0.05, ***p < 0.001, Student’s t-test.

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