Fig. 1: VHL-deficient cells more sensitive to glutamine metabolism. | Cell Death & Disease

Fig. 1: VHL-deficient cells more sensitive to glutamine metabolism.

From: VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway

Fig. 1

A, B VHL-proficient (Caki-2) and VHL-deficient (786-O) cells were cultured in complete medium or medium absence of glutamine. A Cell growth was determined by CCK8 and normalized to the corresponding cell type (Caki-2 or 786-O) grown in complete medium. B Representative phase-contrast photomicrograph was used to monitor cell survival after cultured for 96 h. C Caki-2 and 786-O cells were cultured for 48 h by complete medium with or without BPTES (10 μM)/EGCG (25 μM). Cell viability was determined by CCK8 assay. Error bars represent mean ± SD (n = 3). *P < 0.05, **P < 0.01. Student’s t test compared treated groups with corresponding control cells.

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