Fig. 8: SIRT4-induced apoptosis in a p38-MAPK dependent manner. | Cell Death & Disease

Fig. 8: SIRT4-induced apoptosis in a p38-MAPK dependent manner.

From: VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway

Fig. 8

A Caki-2 and 786-O cells with SIRT4 overexpression were performed by western blot to analyze Akt or p38-MAPK pathway. Densitometric analysis was quantified on the right panel. B Cells with stable SIRT4 overexpression were treated by Akt (5 μM) or p38-MAPK antagonist (25 μM) for 24 h followed by western blot. Quantification of HO-1 is shown underneath. C Effects of Akt or p38-MAPK antagonist treatment on ROS generation in control and SIRT4 overexpression cells. A summary of the quantification analysis (below panel) is shown. D Effects of p38-MAPK antagonist treatment on apoptosis in control and SIRT4 overexpression cells. E A proposed model illustrating the distinct signaling components triggered by SIRT4. Error bars represent SD (n = 3). *P < 0.05, **P < 0.01, ns: no significance.

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