Fig. 4: Loss of puma rescues mdm2-null induced apoptosis at early embryonic stage.
From: Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis
A Anti-active Caspase-3 staining on 12, 15, 18, and 21 hpf mdm2+/+ and mdm2−/− zebrafish embryos. B qRT-PCR analysis of puma and noxa in 15 hpf mdm2+/+, mdm2 siblings (mdm2+/+ and mdm2+/−) and mdm2−/− zebrafish embryos. n = 3 from ~30 pooled embryos per sample. Bars represent mean ± SEM. **p < 0.01. C Anti-active Caspase-3 staining on 12, 15, 18, and 21 hpf mdm2+/+ bbc3−/− and mdm2−/− bbc3−/− zebrafish embryos. D Gross images of 15, 18, and 21 hpf mdm2+/+, mdm2−/−, and mdm2−/− bbc3−/− zebrafish embryos.
