Fig. 1: Hsa_circ_0110389 is up-regulated in gastric cancer tissues and predicts poor prognosis. | Cell Death & Disease

Fig. 1: Hsa_circ_0110389 is up-regulated in gastric cancer tissues and predicts poor prognosis.

From: Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

Fig. 1

A Heat map of RNA-seq analysis between GES-1-T and GES-1-N cells. The asterisk indicates hsa_circ_0110389. B Schematic illustration of hsa_circ_0110389 formation via the circularization of exons in SORT1 gene. Sanger sequencing conformed the head-to-tail splicing of hsa_circ_0110389. C Expression of hsa_circ_0110389 in different cell lines. D The gel electrophoresis validated the existence of hsa_circ_0110389. Divergent primers amplified hsa_circ_0110389 in cDNA but not in gDNA. GAPDH was used as a linear control. E Expression of hsa_circ_0110389 and SORT1 mRNA in both HGC-27 and KATO III cells was detected by qRT-PCR in the presence or absence of RNase R. F Hsa_circ_0110389 was mainly located in the cytoplasm by nuclear-cytoplasmic fractionation assay. G Relative expression of hsa_circ_0110389 in 110 paired GC tissues and adjacent normal tissues. H, I Kaplan–Meier survival analysis (log-rank test) showed that GC patients with high hsa_circ_0110389 expressions have a lower disease-free survival and overall survival than these with low hsa_circ_0110389 expressions (P < 0.05). All data are presented as mean ± SD. ***P < 0.001.

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