Fig. 3: Hsa_circ_0110389 acts as a molecular sponge for miR-127-5p and miR-136-5p. | Cell Death & Disease

Fig. 3: Hsa_circ_0110389 acts as a molecular sponge for miR-127-5p and miR-136-5p.

From: Circular RNA hsa_circ_0110389 promotes gastric cancer progression through upregulating SORT1 via sponging miR-127-5p and miR-136-5p

Fig. 3

A Bioinformatics analysis showed potential binding interaction between hsa_circ_0110389 and different miRNAs, and RNA pull-down results showed that hsa_circ_0110389 may directly bind with miR-127-5p, miR-136-5p and miR-148a-5p. B Schematic illustration of the sequences of wild type hsa_circ_0110389 (circ-WT) and mutant hsa_circ_0110389 (circ-Mut) in the binding sites of miR-127-5p and miR-136-5p. C, D Relative luciferase activities were detected in GC cells after transfecting luciferase reporter plasmids with wild type or mutant of hsa_circ_0110389 with miR-127-5p/miR-136-5p or negative control. mim, mimic. E, F FISH for subcellular localization of hsa_circ_0110389 and miR-127-5p or miR-136-5p in HGC-27 and KATO III cells. G, H Expression of miR-127-5p (G) or miR-136-5p (H) in 110 paired GC tissues and adjacent normal tissues. I, J Correlation between miR-127-5p (I) or miR-136-5p (J) and hsa_circ_0110389 expression in 110 paired GC tissues. The correlation was measured by Pearson correlation analysis. All data are presented as mean ± SD. **P < 0.01, ***P < 0.001.

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