Fig. 4: Hsa_circ_0110389 promotes the proliferation, migration and invasion of GC cells via miR-127-5p and miR-136-5p.

A The expression of miR-127-5p or miR-136-5p was significantly increased in HGC-27 and KATO III cells after transfected with hsa_circ_0110389 shRNA and then decreased when HGC-27 and KATO III cells were co-transfected with miR-127-5p or miR-136-5p inhibitors. B The expression of miR-127-5p or miR-136-5p was obviously decreased in AGS and NC-N87 cells after transfected with hsa_circ_0110389 overexpression plasmids and then increased when AGS and NC-N87 cells were co-transfected with miR-127-5p or miR-136-5p mimics. C, E, G miR-127-5p or miR-136-5p inhibitors effectively reversed the hsa_circ_0110389 knockdown induced suppression of proliferation and growth in HGC-27 and KATO III cells by CCK-8 assay (C), EdU staining (E) or colony formation assay (G). D, F, H miR-127-5p or miR-136-5p mimics effectively reversed the hsa_circ_0110389 overexpression induced promotion of proliferation in AGS and NCI-N87 cells by CCK-8 assay (D), EdU staining (F) or colony formation assay (H). *vs sh-NC/pCD5-NC group, #vs inh-NC/mim-NC group. I Wound healing results showed that the promoting effect of hsa_circ_0110389 knockdown on migration of HGC-27 and KATO III cells was blocked by miR-127-5p or miR-136-5p inhibitors. J Wound healing results showed that the suppressed role of hsa_circ_0110389 overexpression in migration of AGS and NCI-N87 cells was reversed by miR-127-5p or miR-136-5p mimics. K Transwell assay results showed that the suppressed role of hsa_circ_0110389 silencing in invasion of HGC-27 and KATO III cells was reversed by miR-127-5p or miR-136-5p inhibitors. L Transwell assay results showed that the promoting effect of hsa_circ_0110389 overexpression on invasion of AGS and NCI-N87 cells was blocked by miR-127-5p or miR-136-5p mimics. inh inhibitor, mim mimic. *vs sh-NC/pCD5-NC group, #vs inh-NC/mim-NC group. All data are presented as mean ± SD. **p < 0.01, ***p < 0.001, ^##p < 0.01, ^###p < 0.001.